G002 Transfer of rolf S3-S4 linker to hERG eliminates activation gating but spares inactivation
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چکیده
منابع مشابه
Modulation of the Shaker K+Channel Gating Kinetics by the S3–S4 Linker
In Shaker K(+) channels depolarization displaces outwardly the positively charged residues of the S4 segment. The amount of this displacement is unknown, but large movements of the S4 segment should be constrained by the length and flexibility of the S3-S4 linker. To investigate the role of the S3-S4 linker in the ShakerH4Delta(6-46) (ShakerDelta) K(+) channel activation, we constructed S3-S4 l...
متن کاملPeriodic perturbations in Shaker K+ channel gating kinetics by deletions in the S3-S4 linker.
Upon depolarization positive charges contained in the transmembrane segment S4 of voltage-dependent channels are displaced from the cytoplasmic to the external milieu. This charge movement leads to channel opening. In Shaker K+ channels four positively charged arginines in the S4 domain are transferred from the internal to the external side of the channel during activation. The distance travele...
متن کاملThe S4–S5 Linker Acts as a Signal Integrator for hERG K+ Channel Activation and Deactivation Gating
Human ether-à-go-go-related gene (hERG) K(+) channels have unusual gating kinetics. Characterised by slow activation/deactivation but rapid inactivation/recovery from inactivation, the unique gating kinetics underlie the central role hERG channels play in cardiac repolarisation. The slow activation and deactivation kinetics are regulated in part by the S4-S5 linker, which couples movement of th...
متن کاملCritical roles of the S3 segment and S3-S4 linker of repeat I in activation of L-type calcium channels.
Each of the four repeats (or motifs) of voltage-gated ion channels is thought to contain six transmembrane segments (S1-S6). Mutational analyses indicate that S4 functions as a voltage sensor and that the S5, S6, and S5-S6 linker contribute to formation of the ion pore. However, little information exists regarding the functional role(s) of the amino-terminal portion (S1-S3-S4 linker) of the rep...
متن کاملRole of the S3-S4 Linker in Shaker Potassium Channel Activation
Structural models of voltage-gated channels suggest that flexibility of the S3-S4 linker region may be important in allowing the S4 region to undergo large conformational changes in its putative voltage-sensing function. We report here the initial characterization of 18 mutations in the S3-S4 linker of the Shaker channel, including deletions, insertions, charge change, substitution of prolines,...
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ژورنال
عنوان ژورنال: Archives of Cardiovascular Diseases
سال: 2009
ISSN: 1875-2136
DOI: 10.1016/s1875-2136(09)72277-6